By Tara L. Fulton (auth.), Beth Shapiro, Michael Hofreiter (eds.)
Research into historical DNA begun greater than 25 years in the past with the book of brief mitochondrial DNA series fragments from the quagga, an extinct relative of the zebra. old DNA study particularly won momentum following the discovery of PCR, which allowed hundreds of thousands of copies to be made up of the few final DNA molecules preserved in fossils and museum specimens. In Ancient DNA: equipment and Protocols professional researchers within the box describe the various protocols which are now common to check historic DNA. those comprise directions for developing an old DNA laboratory, extraction protocols for quite a lot of various substrates, information of laboratory strategies together with PCR and NGS library practise, and recommendations for applicable analytical methods to make feel of the sequences got. Written within the hugely profitable Methods in Molecular Biology™ sequence layout, chapters comprise introductions to their respective themes, lists of the required fabrics and reagents, step by step, effectively reproducible laboratory protocols, and key pointers on troubleshooting and heading off recognized pitfalls.
Authoritative and functional, Ancient DNA: tools and Protocols seeks to help scientists within the additional research of old DNA and the methodological methods in historic research.
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